Pharmaceuticals
9th Sep 2025

Preventing contamination in pharmaceutical fermentation processes with filtration

When a single unwanted microorganism can destroy weeks of work and cost millions, preventing contamination in pharmaceutical fermentation processes is paramount. Filtration provides the most reliable defence against microbial contamination, ensuring sterility across all process inputs – from sterile gas and water systems to chemical additions and antifoams.

What is microbial contamination?

In the context of pharmaceutical fermentation, microbial contamination refers to the presence of any unwanted microorganism that interferes with the production process.

Unlike traditional manufacturing, pharmaceutical fermentation processes deliberately cultivate specific microorganisms – such as genetically modified E. coli for insulin production. This makes controlling contamination in pharmaceutical fermentation processes uniquely challenging as you must eliminate all microorganisms except the intended production strain.

"It [microbial contaminants] can be likened to having weeds in your garden. You're trying to grow your lovely flowers, and you don't want these extra plants in there.

— David Ridealgh, Head of Marketing, Amazon Filters

Manufacturers often genetically modify production microbes for specificity, ensuring they produce only the target compound. For example:

  • Insulin
  • Antibiotics
  • Enzymes

Common sources of contamination in fermentation processes

Utilities

In pharmaceutical fermentation, risk often stems from utilities such as:

  • Sterile gas
  • Water
  • Steam
  • Chemical additions

Sterile gas presents the greatest challenge for pharmaceutical manufacturers. Large-scale fermenters, ranging from 10-200m³, require substantial air volumes for aerobic processes. The greater the volume, the harder it is to ensure sterility. But of course, any contamination can have catastrophic effects. Bacteriophage contamination, for instance, can completely shut down your fermentation processes.

Water and steam systems must maintain sterility throughout distribution networks. Both process water for nutrient solutions and steam for sterilisation can introduce contaminants like Pseudomonas.

Raw materials and chemical additions

Despite their aggressive nature, chemical inputs can harbour microbial spores – including pH-adjusting acids and bases. Even high-concentration solutions like phosphoric acid and ammonia can contain spores that survive and activate when introduced to nutrient-rich fermentation broth.

These additions become particularly critical during active fermentation when the system receives multiple inputs over extended periods.

Personnel and equipment

Fermenters, pipework, valve housings, and associated systems need comprehensive sterilisation processes to prevent contamination. Residual condensate, cold spots, and inadequate drainage can create contamination reservoirs, compromising sterility. This makes system design crucial. For example, implementing proper steam traps, drainage, and validated sterilisation processes.

Processes that require manual operator intervention can lead to contamination. However, careful facility design can minimise risk. This includes measures like using closed systems to reduce operator exposure and establishing appropriate training protocols to ensure everyone's familiar with best practices.

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Contamination control challenges

Multiple factors make contamination control in pharmaceutical fermentation particularly difficult – from operational complexity and risks to the sensitivity of fermentation organisms.

Complexity of fermentation environments

High-nutrient media formulations create ideal conditions for microbial growth. Contaminating organism can proliferate as rapidly as the intended production strains, disrupting the process. Extended process durations – often weeks rather than hours – provide multiple opportunities for contaminants to enter fermentation processes.

The scale of modern fermentation presents additional complexity. Sterilising 100 cubic metres of broth requires robust steam systems operating at elevated temperatures (135-142°C). However, achieving uniform heat distribution within such large volumes is challenging.

Sensitivity of fermentation organisms

Production strains, particularly genetically modified variants, are often less robust than unmodified, wild-type organisms. The increased sensitivity means naturally occurring contaminating organisms frequently outcompete production strains, leading to rapid process deterioration.

Distinguishing intended microorganisms from closely related contaminants presents a significant operational challenge. Traditional detection methods may lack sufficient sensitivity or speed to prevent contamination before irreversible damage occurs.

As fermentation progresses, you’ll add nutrients and other inputs. Each addition is an opportunity for contamination to enter your processes.

Operational and equipment risks

Human error is a persistent risk factor in pharmaceutical fermentation, particularly during manual interventions such as sampling, nutrient additions, or equipment adjustments. Best practice recommends minimising the frequency with which you open your system and conducting all operations through a closed fermentation filtration system.

Then there are equipment failure risks. These extend beyond obvious mechanical breakdowns to include subtle issues like:

  • Incomplete sterilisation cycles
  • Compromised filter integrity
  • Inadequate temperature control

These failures may not manifest immediately, allowing contamination to establish before detection.

5 ways microbial contamination impacts pharmaceutical production

1. Reduced product yield

Even seemingly minor contamination can significantly impact productivity through metabolite interference. Some contaminants produce byproducts, like lactic acid, that inhibit intended microorganism growth, reducing yields or causing complete process failure in the worst cases.

2. Compromised product quality and safety

Contamination can alter product molecular structures or introduce unwanted compounds requiring additional downstream purification. For products like insulin, maintaining exact molecular structure specifications is critical for patient safety and regulatory compliance. Contamination can create unwanted products that require extra purification downstream, increasing both costs and complexity.

3. Batch loss and associated costs

Losing an entire product batch can have a significant impact on your bottom line – especially for things like insulin, which can take up to 30 days to process. A single contaminated 100-cubic-metre fermenter represents millions in wasted raw materials, spent utilities, and missed opportunities.

4. Regulatory non-compliance

Contamination events trigger mandatory reporting requirements and potential regulatory investigations. Repeated incidents may result in facility inspections, consent decrees, or production restrictions.

5. Operational inefficiency

The obvious response to any contamination is to investigate the cause. This requires a significant investment of laboratory resources and technical personnel. And, once you've concluded your investigation, you'll need to decontaminate your facility. These activities divert resources from productive activities and lead to long periods of operational downtime, which has a direct impact on revenue.

The role of filtration in contamination control

Filtration provides the most reliable method for ensuring sterility of all inputs entering fermentation systems. Unlike heat sterilisation, which may not achieve complete microbial destruction and can degrade chemical additives, sterile filtration offers consistent performance without compromising product quality.

Industry experts advocate using filtration instead of heat sterilisation wherever possible, particularly for smaller chemical additions during fermentation. This approach recognises that, while heat sterilisation may work for large initial volumes, it isn't always reliable. Filtration offers more certainty.

"It's not practical to filter 100 m³ of broth, so you sterilise it with steam once it's in the fermenter. But for the additives added over time – like more broth, nutrients, antifoams – filtration is more reliable and easier to manage."

— David Ridealgh, Head of Marketing, Amazon Filters

Types of filtration for fermentation

Effective contamination control requires a tailored approach to each process input and stage. Different types of filtration addresses specific contamination risks while considering factors like chemical compatibility and operating conditions.

Pre-filtration

Pre-filtration systems remove larger particles and biological contaminants before sterile filtration stages, extending membrane life and ensuring consistent performance. For process streams containing variable particle loads, depth filters are your best bet.

Sterile filtration

Sterile filtration using 0.2-micron absolute-rated membranes reduces bioburden reduction across process inputs:

  • Sterile gas
  • Antifoam
  • Acids
  • Bases
  • Water

Polyethersulfone (PES) membranes offer broad chemical compatibility for most fermentation applications, while polytetrafluoroethylene (PTFE) membranes handle aggressive chemicals and elevated temperatures.

Antifoam filtration

Heat sterilisation can alter the viscosity and effectiveness of antifoams, like polypropylene glycol (PPG). Filtration at point-of-use with 0.2-micron PES membranes ensures sterility without impacting performance.

Vent and gas filtration

Sterile gas filtration demands high-flow, low-pressure-drop solutions. Sterilising temperatures for large equipment often reach 135–142°C, wich necessitates the use of specialised filters, like PTFE membranes, that are capable of withstanding these extreme conditions.

Because the volumes are so high, minimising pressure drops becomes essential for controlling operational costs. This requires careful attention to both filter and housing design.

Filter material selection and compatibility

Material compatibility is crucial when filtering aggressive chemicals like phosphoric acid and ammonia. Even high-concentration solutions can contain spores that require filtration. Polypropylene-supported PES membranes have excellent chemical resistance and maintain consistent filtration performance under challenging conditions.

In pharmaceutical fermentation applications, housing design directly impacts filtration performance. As such, your filter housings must be fully sterilisable and incorporate proper drainage systems to eliminate dead legs. These design details prevent contamination reservoirs that can compromise even your filtration system.

For sterile gas applications, specialised housings with plenum chambers reduce pressure drops across large-volume gas filters. This ensures both effective filtration and energy efficiency for the massive air volumes your pharmaceutical fermentation processes require.

Protecting pharmaceutical fermentation through strategic filtration

Effective contamination control in pharmaceutical fermentation requires matching the right sterilisation method to each specific application. This means combining steam sterilisation for massive broth volumes with point-of-use filtration for ongoing additions throughout the process.

This hybrid approach recognises that different contamination risks require different solutions. While heat sterilisation handles large-scale requirements, filtration provides the consistency essential for maintaining sterile conditions during extended production cycles.

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